26 No. 6
Reference Methods, Standards, and Applications of Photoluminescence
Measurements of the fluorescence properties of molecules are critical to the development of an understanding of the photophysics and photochemistry of the singlet excited state(s) of the species. A reliable and standard methodology is also required if published data are to be used to the photochemical community at large. Methods for fluorescence-decay analysis are also an integral part of the determination of the photophysical parameters of the species, as these time-resolved fluorescence properties are useful in spectroscopy, kinetics, energy transfer, analytical applications, and in characterization of chemical, biological, and physical systems which emit light.
The purpose of this new project is to review and update the two previous IUPAC documents covering this topic, namely “Reference Materials for Fluorescence Measurement,” Pure Appl. Chem., 60(7), 1107–1114 (1988) and “Recommended Methods for Fluorescence Decay Analysis,” Pure Appl. Chem., 62(8), 1631–1648 (1990). The project, which will include advances registered since the earlier publication, will emphasize newly developed reference materials and methods.
The fields to be covered by the project include calibration (spectra, quantum yields, decay times); procedures (steady-state, time-resolved methods including ultrafast techniques, single-molecule studies); and applications to biological (cells, organelles, membranes) and other microorganized systems (gels, films, solids, and, in general, complex environments, usually contaminated by light scattering).
A task group for the project, including nearly 20 experts from different specialties and countries, was formed and leaders for the different subtasks were nominated. The photochemical community is encouraged to participate by joining any of the subgroups. Communication will be performed through the project Web page, and a vast dissemination plan will be also devised. The project began mid-year in 2004 and will end at the end of 2006. Before final documents are written, advance reports and document drafts will be issued.
For more information, contact the Task Group Chairmen, Enrique San Román <email@example.com> or Fred Brouwer <firstname.lastname@example.org>.
last modified 10 November 2004.
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