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Pure Appl. Chem. 75(2-3), 309-316, 2003

Pure and Applied Chemistry

Vol. 75, Issue 2-3

Exploitation of proteomics strategies in protein structure-function studies

G. Marino, P. Pucci, L. Birolo, and M. Ruoppolo

1Dipartimento di Chimica Organica e Biochimica, Facoltà di Scienze Biotecnologiche, Università di Napoli “Federico II”, Napoli, Italy; 2CEINGE, Biotecnologie Avanzate, scrl, Napoli, Italy; 3Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II”, Napoli, Italy

Abstract: Mass spectrometry plays a central role in structural proteomics, particularly in highly intensive structural genomics projects. This review paper reports some examples taken from recent work from the authors' laboratory and is aimed at showing that modern proteomics strategies are instrumental in the integration of structural genomic projects in fields such as: (i) protein–protein interactions, (ii) protein-DNA interactions, (iii) protein–ligand interactions, and (iv) protein-folding intermediates.

*Pure Appl.Chem. 75, 141419 (2003). An issue of reviews and research papers based on lectures presented at the 23rd IUPAC International Symposium on the Chemistry of Natural Products, Florence, Italy, 28 July 2 August 2002.


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