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Project

Analytical Chemistry Division (V)

 

COMMISSION ON SEPARATION METHODS IN ANALYTICAL CHEMISTRY (V.3)

Number: 530/12/97

Title: Hold-up volume in column chromatography

Coordinator(s): J. A. Garcia-Dominguez

Completion Date: 2001 - project completed

Objective:
Although it is a fundamental concept in chromatography the hold-up or void volume) of a column system has been defined in different ways in different systems. A greater difficulty is lack of agreement on the methods that can be used to determine the measurement. The aim of the project is to provide a critical review of the background and theory of the meaning of the terminology and to critically compare the different methods of measurement and of the significance of the values that they generate.

Progress:
A final draft has circulated to Commission members and submitted to the Division. Some of the terms have been coordinated with project 530/13/97.

A revised document is submitted to public review comments until 28 February 2001
> see provisional recommendations

A combined manuscript entitled 'Retention Parameters in Chromatography. Part A. The Hold-Up Volume Concept in Column Chromatography. Part B. Retention Parameters in Gas Chromatography.' is published in Pure Appl. Chem. 73(6), pp. 969-992, 2001

Last Update: 24 September 2001

 

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