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Pure Appl. Chem. 76(2), 321-333, 2004

Copper(II) proteins are amenable for NMR investigations

I. Bertini and R. Pierattelli

Department of Chemistry and Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino (Florence), Italy

Abstract: The possibility of investigating copper proteins by nuclear magnetic resonance is here treated. It is shown that the solution structure and dynamic information can be obtained on Type I Cu(II) proteins, which have a relatively short electron relaxation time (t_S = 10^-10 s). The experimental approach, in this case, is routine, although tailored for fast-relaxing nuclei.

In the case of Type II Cu(II) proteins, when the electron relaxation times are larger (t_S = 10^-8 - 10^-9 s) and proton linewidths are broadened beyond detectable limits, heteronuclear ¹³C spectra with direct detection can be used and the solution structure can again be obtained.

In this review, it is shown that Cu(II) proteins are now amenable for NMR investigation, the size being the only limit as in diamagnetic proteins.

*Plenary lecture presented at the 2nd Santa Mar�a Workshop on Chemistry Devoted to Bioinorganic Chemistry, Santa Mar�a del Mar, Havana, Cuba, 7-11 July 2003. Other presentations are published in this issue, pp. 321-388.

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